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KMID : 0364820150510010021
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Korean Journal of Microbiology
2015 Volume.51 No. 1 p.21 ~ p.30
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Comparison of community structure of sulfate reducing bacteria in rice paddy and dry farming soils
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Lee Jung-Bae
Park Kyeong-Ryang
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Abstract
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The goal of this study was to identify relationships between the composition of sulfate reducing bacterial assemblages and terminal restriction fragment length polymorphism (T-RFLP) patterns in rice paddy and dry farming soils. Samples of organic farming soils, conventional farming soils, and dry field farming soils were collected in August and November. Analyses of the soil chemical composition revealed similar total nitrogen, total carbon and total inorganic phosphorus levels; however, the moisture content and total carbon were higher than in the other soils in both August and November, respectively. Sulfate reducing bacteria utilizing lactic acid were more widely distributed than those that used acetic acid, and the number of sulfate reducing bacteria in organic farming soil was most abundant. Phylogenetic analysis based on 181 clones revealed that most showed low similarity with cultured sulfate reducing bacteria, but more than 90% similarity with an uncultured sulfate reducing bacteria isolated from the environment. T-RFLP analysis revealed that fragments of 91, 357, 395, and 474 bp were most common, and the community structure of sulfate reducing bacteria changed seasonally.
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KEYWORD
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dry farming soil, phylogenetic tree, rice paddy soil, sulfate reducing bacteria, T-RFLP
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